Transcription_Factor | Comment |
---|---|
Promoter P1 | Promoter P1 controls ST6Gal I expression in liver and intestinal epithelium {Wang_X/Lau_JT.1993.JBC} ; {Vertino-Bell_A/Lau_JT.1994.DB} . P1 activity is modulated by liver-enriched transcription factors HNF-1, DBP, and LAP {Svensson_EC/Paulson_JC.1992.JBC} as well as by glucocorticoid {Wang_XC/Lau_JT.1989.JBC} |
Promoter P2 | Promoter P2 region containing AP2, NF-kB, and TATA transcriptional start sites controls ST6Gal I expression in mature B cells {Lo_NW/Lau_JT.1996.BBRC} |
Promoter P3 | Promoter P3 was shown to control expression of hST6Gal I gene during HL-60 differentiation. The activity is modulated by Sp-1 and Oct-1 {Taniguchi_A/Matsumoto_K.2000.GLYCO} |
Substrate | Comment |
---|---|
Terminal GalBeta1,4GlcNAc containing N-linked glycoproteins | Terminal GalBeta1,4GlcNAc containing N-linked glycoproteins, such as, prolactin {Manzella_SM/Baenziger_JU.1997.JBC} |
lactoferrin | {Coddeville_B/Spik_G.1992.CR} |
fat globule membrane CD36 | {Nakata_N/Kobata_A.1993.BIOCH} |
HB-6 | {Bast_BJ/Tedder_TF.1992.JCB} |
CD75s (formerly CDw75 and CDw76) | {Bast_BJ/Tedder_TF.1992.JCB} |
Various milk oligosaccharides | {Gyorgy_P/Zilliken_F.1974.EJB} |
Species_or_Strain | Supplier | Comment |
---|---|---|
Homozygous ST6Gal deficient female mice | Dr. Jamey D. Marth Howard Hughes Medical Institute San Diego, CA |
Homozygous ST6Gal I deficient female mice in C57BL/6 background {Hennet_T/Marth_JD.1998.PNASU} |
Reagent | Supplier | Comment |
---|---|---|
alpha2,6-(N)-sialyltransferase, from rat liver | Calbiochem ( 566217 ) | |
alpha2,6-(N)-sialyltransferase, rat, recombinant, Spodoptera frugiperda | Calbiochem ( 566222 ) | |
Sialyltransferase | Sigma ( S2769 ) |